庫號：D64412

直鏈淀粉/支鏈淀粉[膠淀粉]檢測試劑盒
英文名: Amylose/Amylopectin Assay Kit
貨號: K-AMYL
規(guī)格: 100 assays per kit
直鏈淀粉/支鏈淀粉[膠淀粉]檢測試劑盒
英文名：Amylose/Amylopectin Assay Kit
貨號：K-AMYL
規(guī)格：100 assays per kit
分析物意義: 影響消化速率和淀粉利用的成分比例
Megazyme檢測試劑盒點: 試劑盒新穎，試劑穩(wěn)定
For the measurement of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure. Content:100 assays per kit
Colourimetric method for the determination of Amylose and
Amylopectin in cereals, food and feed
Principle:
(Con A)
(1) Soluble starch (amylose + amylopectin) →
amylose + amylopectin-Con A
(soluble) (precipitate)
(α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + H2O
(α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H2O → D-glucose
Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 120 min
Detection limit: Amylose 5-95% of total starch content
Application examples:
Cereal starches, flours, pure starches and foods